other:Article Title: METTL14‐Induced M 6 A Methylation Increases G6pc Biosynthesis, Hepatic Glucose Production and Metabolic Disorders in Obesity
Article Snippet: METTL3 ABclonal A8370 1:2000 METTL14 Sigma HPA038002 1:2000 WTAP ABclonal A14695 1:1000 m6A Cell Signaling Technology 56593 1:2000 pAKT (pThr308) Cell Signaling Technology 2965 1:2000 pAKT (pSer473) Cell Signaling Technology 4060 1:2000 AKT Cell Signaling Technology 2920 1:2000 pCREB Cell Signaling Technology 9198 1:2000 CREB Cell Signaling Technology 4820 1:2000 G6PC ABclonal A21168 1:1000 p85 Home made N/A 1:5000 Lamin A/C Cell Signaling Technology 4777 1:2000 ACC1 Cell Signaling Technology 3676 1:2000 FASN Cell Signaling Technology 3180 1:2000 ACLY Cell Signaling Technology 4332 1:2000 SCD1 Cell Signaling Technology 2794 1:2000 HA Home made N/A 1:2000 Flag Sigma F1804 1:5000 FTO Abcam Ab94482 1:2000 ALKBH5 Proteintech Group 16837-1-AP 1:1000 YTHDF1 ABclonal A23773 1:2000 YTHDF2 Cell Signaling Technology 71283 1:2000 YTHDF3 ABclonal A8395 1:2000
Article Title: LAMC1 Aggravates Diabetic Retinopathy Through PI3K/AKT Signaling-Regulated Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells
Article Snippet: LAMC1 Cell Signaling Technology (92921) 1:1000 E-cadherin ABclonal (A20798) 1:1000 N-cadherin Proteintech (22018-1-AP) 1:2000 Vimentin Abcam (ab92547) 1:1000 Snail ABclonal (A11794) 1:1000 PI3K Cell Signaling Technology (4292) 1:1000 p-PI3K Affinity Biosciences (AF3242) 1:1000 Jo ur na l P re -p ro of AKT Cell Signaling Technology (9272) 1:1000 p-AKT Cell Signaling Technology (9271) 1:1000 GAPDH ABclonal (A19056) 1:10000 Goat Anti-Rabbit IgG H&L (HRP) ABclonal (AS014) 1:10000
Article Title: Supporting Information for Regression of post-prandial cardiac hypertrophy in Burmese pythons is mediated by FoxO1
Article Snippet: Antibody Vendor Product Number Dilution p-FoxO1/3 (T24) Cell Signaling Technology 2599 1:1000 LC3B Cell Signaling Technology 2775 1:1000 p-mTOR (S2448) Cell Signaling Technology 2971 1:1000 mTOR Cell Signaling Technology 4517 1:1000 p-Akt (S473) Cell Signaling Technology 9271 1:1000 Akt Cell Signaling Technology 2920 1:1000 GAPDH Cell Signaling Technology 2118 1:1000 FoxO1 Cell Signaling Technology 14952 1:1000 p-ULK1 (S555) Cell Signaling Technology 5869 1:1000 p-ULK1 (S757) Cell Signaling Technology 6888 1:1000 Puromycin Millipore Sigma 12D10 1:1000 Pan-ubiquitin Cytoskeleton AUB01 1:1000 HRP Anti-Rb IgG Cell Signaling Technology 7074 1:2000 HRP Anti-MS IgG Cell Signaling Technology 91196 1:2000 IR-Dye 800CW LICOR 926-32211 1:4000 IR-Dye 680RD LICOR 926-68070 1:4000 Figure S1.
Article Title: GTP State-Selective Cyclic Peptides Ligands of K-Ras(G12D) Block its Interaction with Raf
Article Snippet: Target Supplier Identifier Dilution Pan-Ras abcam 108062 1:5000 P-ERK [T202/Y204] Cell Signaling Technology 9101 1:1000 Total ERK Cell Signaling Technology 4695 1:1000 P-S6 [S240/S244] Cell Signaling Technology 5364 1:2000 S6 Cell Signaling Technology 2217 1:1000 P-AKT [S473] Cell Signaling Technology 4060 1:1000 AKT Cell Signaling Technology 2920 1:1000 Actin Proteintech 60008-1-Ig 1:50000
Article Title: LAMC1 Aggravates Diabetic Retinopathy Through PI3K/AKT Signaling-Regulated Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells
Article Snippet: LAMC1 Cell Signaling Technology (92921) 1:1000 E-cadherin ABclonal (A20798) 1:1000 N-cadherin Proteintech (22018-1-AP) 1:2000 Vimentin Abcam (ab92547) 1:1000 Snail ABclonal (A11794) 1:1000 PI3K Cell Signaling Technology (4292) 1:1000 p-PI3K Affinity Biosciences (AF3242) 1:1000 AKT Cell Signaling Technology (9272) 1:1000 p-AKT Cell Signaling Technology (9271) 1:1000 GAPDH ABclonal (A19056) 1:10000 Goat Anti-Rabbit IgG H&L (HRP) ABclonal (AS014) 1:10000 Jo ur na l P re -p ro of
Western Blot:Article Title: PFKFB2 Is Pivotal for Metabolic Flexibility and Differential Glucose Utilization
Article Snippet: .. Antibodies Used for Western Blot Analysis Antibody against Producer/product number Primary dilution Secondary dilution Akt Cell Signaling Technology (CST): 2920S 1:1000 1:3000 Phospho- Akt (serine 473) CST: 4060S 1:1000 1:3000 GFAT1 CST: 5322S 1:500 1:2000 OGA/MGEA5 Proteintech:14711- 1- AP 1:1000 1:3000 OGT Sigma: O6264 1:1000 1:3000 O- linked N- acetylglucosamine Rabbit mAb CST: 82332S 1:1000 1:3000 RL2 Novus: NB300- 524 1:500 (WB) 1:1000 (immunoprecipitation) 1:2000 (WB) 1:3000 (immunoprecipitation) PDH CST: 3205S 1:1000 1:3000 Phospho- PDH (serine 293) CST:31866S 1:1000 1:3000 PDK1 Enzo Life Sciences: ADI- KAP- PK112 1:250 1:2000 PDK2 Santa Cruz: sc- 100 534 1:500 1:2000 PDK4 Biosynthesis, Inc.19 1:750 1:3000 Sp1 Sigma: 07–645 1:1000 1:3000 Akt indicates protein kinase B; GFAT1, glutamine fructose 6- phosphate amidotransferase; OGA, O- GlcNAcase; OGT, O- GlcNAc transferase; PDH, pyruvate dehydrogenase; PDK, pyruvate dehydrogenase kinase; Sp1, specificity protein 1; and WB, western blot. ..
Immunoprecipitation:Article Title: PFKFB2 Is Pivotal for Metabolic Flexibility and Differential Glucose Utilization
Article Snippet: .. Antibodies Used for Western Blot Analysis Antibody against Producer/product number Primary dilution Secondary dilution Akt Cell Signaling Technology (CST): 2920S 1:1000 1:3000 Phospho- Akt (serine 473) CST: 4060S 1:1000 1:3000 GFAT1 CST: 5322S 1:500 1:2000 OGA/MGEA5 Proteintech:14711- 1- AP 1:1000 1:3000 OGT Sigma: O6264 1:1000 1:3000 O- linked N- acetylglucosamine Rabbit mAb CST: 82332S 1:1000 1:3000 RL2 Novus: NB300- 524 1:500 (WB) 1:1000 (immunoprecipitation) 1:2000 (WB) 1:3000 (immunoprecipitation) PDH CST: 3205S 1:1000 1:3000 Phospho- PDH (serine 293) CST:31866S 1:1000 1:3000 PDK1 Enzo Life Sciences: ADI- KAP- PK112 1:250 1:2000 PDK2 Santa Cruz: sc- 100 534 1:500 1:2000 PDK4 Biosynthesis, Inc.19 1:750 1:3000 Sp1 Sigma: 07–645 1:1000 1:3000 Akt indicates protein kinase B; GFAT1, glutamine fructose 6- phosphate amidotransferase; OGA, O- GlcNAcase; OGT, O- GlcNAc transferase; PDH, pyruvate dehydrogenase; PDK, pyruvate dehydrogenase kinase; Sp1, specificity protein 1; and WB, western blot. ..
BIA-KA:Article Title: Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models
Article Snippet: Immunofluorescence involved fixation, permeabilization, antibody staining, and imaging via DeltaVision microscopy. .. Tissues and cells were lysed in RIPA or urea buffers, protein quantified via BCA assay, electrophoresed via SDS-PAGE, transferred to nitrocellulose membranes, and probed with antibodies listed: CEP55 CST (D1L4H) Rabbit mAb #81693 1:1000; Cep55 Santa Cruz biotechnology, sc-374051, 1:500; Vinculin Cell Signaling Technology #13901, 1:2000; β-Actin BD Pharmingen#612656, 1:2000; β-Catenin Cell Signaling Technology 9582, 1:1000; pERK1/2 (T202/Y204) Cell Signaling Technology #4370, 1:1000; ERK1/2Cell Signaling Technology #4695, 1:1000; pAKTS473 Cell Signaling Technology #4060, 1:1000; AKT Cell Signaling Technology #9271, 1:1000; MYC (Y69) Abcam #ab32072, 1:1000; Rabbit Secondary (peroxidase) Sigma Aldrich #A0545, 1:5000; Mouse Secondary (peroxidase) Sigma Aldrich #A9044, 1:5000. .. Messenger RNA levels, i.e., batch effect-normalized log2(normalized counts+1) (Illumina HiSeq RNASeqV2), were obtained from the pan-cancer dataset of The Cancer Genome Atlas (TCGA), including 33 cancer types ( ) and processed as previously described ( ).
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